Tsai utilizes a variety of media in a politically engaged, performative practice. At once highly personal yet general in concern, Tsai grounds her self and art practice in a sense of (national / Taiwanese) identity and the consequent implications. Geographical, social and spiritual concerns inform a body of work directed towards activating participation outside the confines of complacent contemplation.
Tsai has had solo exhibitions in Beijing, Bogota, Hong Kong, Mumbai, Paris, Singapore, Sydney, Taipei, and Tokyo, and her projects have been included in various international exhibitions, including "J'en Rêve" at Fondation Cartier, Paris, France (2005), the inaugural Singapore Biennale (2006), "Thermocline of Art: New Asian Waves" at ZKM Center of Art and Media, Karlsruhe (2007), "Traces du Sacré" curated by Jean de Loisy at Centre Pompidou, Paris (2008), "6th Asia Pacific Triennial" (2009), "Taiwan Calling"at Ludwig Museum, Budapest (2010), "Yokohama Triennale" and "Ruhrtriennale" (both in 2011), "Phantoms of Asia" curated by Mami Kataoka at Asian Art Museum of San Francisco and "Boundaries on the Move – A Cross-Cultural Diaologue" at Herzliya Museum (both in 2012). Tsai's works are in the collection of Mori Art Museum in Tokyo, Queensland Art Gallery in Brisbane and Sherman Contemporary Art Foundation in Sydney.
In addition to her art practice, Tsai publishes an artist's journal Lovely Dazetwice annually since 2005, which is in the collection of MoMA Public Library, Pompidou Center's Bibliotheque Kandinsky, Tate Modern Library, and Museum of Contemporary Art in Barcelona Library amongst others.